Occurrence of 9 homologous repeat units in the external spacer region of a nuclear maize rRNA gene unit.
نویسندگان
چکیده
A 14 kb maize DNA fragment carrying nuclear rRNA genes and spacer regions was isolated and characterised by restriction enzyme mapping. A complete 3020 bp long external spacer region was sequenced and revealed 9 tandemly arranged 200 bp long repeat units with high homology. The repeat units lie upstream from two prominent S1 mapping signals. The sequence of a typical repeat unit is compared to a corresponding 130 bp long wheat repeat unit. The possible functional relevance of the repeat units is discussed.
منابع مشابه
Distribution, Fauna and Seasonal Variation of Sandflies, Simultaneous Detection of Nuclear Internal Transcribed Spacer Ribosomal DNA Gene of Leishmania major in Rhombomys opimus and Phlebotomus papatasi, in Natanz District in Central Part of Iran
Background: Zoonotic cutaneous leishmaniasis (ZCL) due to Leishmania major is increasing in many parts of Iran. This disease originally is a disease found in gerbils. Leishmania parasites are transmitted by sandflies that live and breed in gerbil burrows. Nested PCR amplified Leishmania ITS1-5.8S rRNA gene in both main reservoir host “Rhombomys opimus” and in the “Phlebotomus papatasi” main vec...
متن کاملRibosomal gene structure, variation and inheritance in maize and its ancestors.
We have examined the structure of nuclear genes coding for ribosomal RNAs in maize and its wild relatives, the teosintes and Tripsacum. Digestion of the rDNA (genes coding for 18S, 5.8S and 26S RNAs) with 15 restriction endonucleases (with six base pair recognition sites) yields essentially a single map for the approximately 10,000 repeat units within an individual plant or species. Both length...
متن کاملSequence arrangement of the rRNA genes of the dipteran Sarcophaga bullata.
Velocity sedimentation studies of RNA of Sarcophaga bullata show that the major rRNA species have sedimentation values of 26S and 18S. Analysis of the rRNA under denaturing conditions indicates that there is a hidden break centrally located in the 26S rRNA species. Saturation hybridization studies using total genomic DNA and rRNA show that 0.08% of the nuclear DNA is occupied by rRNA coding seq...
متن کاملIndependence of color intensity variation in red flesh apples from the number of repeat units in promoter region of the MdMYB10 gene as an allele to MdMYB1 and MdMYBA
MdMYB10 gene expression results in accumulation of anthocyanin in many tissues including flesh of applefruit. The MdMYB1 and MdMYBA genes are close homologues to MdMYB10 gene and both are responsiblefor red color phenotype in apple fruit skin. In the current study, an apple genome sequence draft analysisindicated that these three genes are located in a unique contig. Further a...
متن کاملOrganization of the Ribosomal RNA Gene Cluster in Lytechinus
The DNA coding for ribosomal RNA from Lytechinus variegatus, an Atlantic sea urchin species, is arranged in repeating units. Each unit is cleaved into four fragments by the restriction endonuclease Eco RI. The same gene region is dissected by the enzyme Sal 1 to yield two fragments. The restriction nuclease Kpn I has one restriction site within each repeat unit, thus generating a single fragmen...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Nucleic acids research
دوره 14 12 شماره
صفحات -
تاریخ انتشار 1986